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Table 2 PCR primers used in this study

From: Transcriptome analysis revealed that a quorum sensing system regulates the transfer of the pAt megaplasmid in Agrobacterium tumefaciens

Targeted gene or region

 

Primer sequencea

P4 cinI

F :

GGCGCAATACCTCAATTGATTG

R :

CATCATGGACGTCGTATCGATC

P4 pAt non coding-region (coord. 36817–37590b)

F :

CATCTCGTTGATCGCAACATGGTCGTT

R :

GATTTGGATGCTTCAACCGAAGATTTATTTGC

P4 pAt non encoding-region (coord. 36950–37353b)

F :

CCGAAAGTAACATTTGATGCCCCAACTGAATTTT

R :

ATAACTGACAGGCATGCGAAAGCCGTC

P4 cinR cinI cinX

F :

TGCTTGTCGTCTGCGCCGTGAGA

R :

CTCAAACACCGCTGGAGCGAACTCA

P4 avhB5 RT-qPCR

F:

CCAAGCAGATCGAGAGCAT

R:

TTGTTGAGCGAGCCATAAAG

P4 avhB11 RT-qPCR

F:

ACGCTTTCCAAAGCATTGAT

R:

CGTGGTTCGGTTGTGAGATA

P4 AGROTU_05920 RT-qPCR

F:

TTGTTGAATGTATGCGGGTT

R:

TATGCGCTGTGGAGATATGG

P4 traA RT-qPCR

F:

CGAGCTGTTCCTCACTACAGT

R:

TTCAATCTTTCCGACAGCACC

P4 cinR RT-qPCR

F:

CGTCGGAGAGTTCGGTTATT

R:

CCTGCAACAAGTCTGTCCC

P4 cinI (internal) RT-qPCR

F:

ATATGGGAAGGCACACGAAT

R:

AAAGGAACCCAAACGCTCTA

P4 cinX RT-qPCR

F:

AATATCGGCAGGAGCATTTC

R:

GCTCATGCTCAAGGTCAAAG

P4 traG RT-qPCR

F:

GATTTCGATTCGACCCACAT

R:

AGCGGGCCTGTATATCTGAG

P4 gyrB RT-qPCR

F:

GATGGCAAGGCCTATGAAAC

R:

CACTTCCAGAAGATCGGACA

P4 blcR RT-qPCR

F:

CGACATTCAACGACCATCTC

R:

CAGCCGATGTAAACGACTTC

  1. aall primer sequences are given in the 5′- > 3 orientations. F forward primer, R reverse primer
  2. bcoordinates on pAt P4 with respect to the position of the first and last base amplified by the primer couples