Identification of oncogenic driver mutations by genome-wide CRISPR-Cas9 dropout screening

Kiessling, Michael K.; Schuierer, Sven; Stertz, Silke; Beibel, Martin; Bergling, Sebastian; Knehr, Judith; Carbone, Walter; de Vallière, Cheryl; Tchinda, Joelle; Bouwmeester, Tewis; Seuwen, Klaus; Rogler, Gerhard; Roma, Guglielmo

doi:10.1186/s12864-016-3042-2

BMC Genomics

Table 1 List of top 20 sgRNAs targeting kinases for HCC-827 cells

From: Identification of oncogenic driver mutations by genome-wide CRISPR-Cas9 dropout screening

Day 14			Day 21			Day 28
Kinase	sgRNA	Log2 fold change	Kinase	sgRNA	Log2 fold change	Kinase	sgRNA	Log2 fold change
EGFR	HGLibA_14637	−5.61	EGFR	HGLibA_14637	−4.88	EGFR	HGLibA_14637	−5.49
NRBP1	HGLibA_39943	−5.37	CHEK1	HGLibA_09418	−4.81	NRBP1	HGLibA_39943	−5.37
TP53RK	HGLibA_58364	−5.08	LTK	HGLibA_34898	−3.67	PDPK1	HGLibA_43219	−4.54
RIPK2	HGLibA_48607	−4.64	SRPK2	HGLibA_54308	−3.45	CDK11A	HGLibA_08724	−3.98
CHEK1	HGLibA_09418	−4.58	CDK11A	HGLibA_08724	−3.39	TBK1	HGLibA_55891	−3.78
PIK3R3	HGLibA_43942	−4.54	WEE1	HGLibA_61525	−3.22	CHEK1	HGLibA_09418	−3.57
WEE1	HGLibA_61525	−4.10	RET	HGLibA_48160	−3.17	WEE1	HGLibA_61525	−3.48
TBK1	HGLibA_55891	−4.06	TBK1	HGLibA_55891	−3.00	CDK2	HGLibA_08756	−3.37
DYRK2	HGLibA_14277	−3.96	NRBP1	HGLibA_39943	−2.88	TP53RK	HGLibA_58364	−2.97
MERTK	HGLibA_36194	−3.78	PHKG1	HGLibA_43699	−2.64	TLK1	HGLibA_56909	−2.82
CLK2	HGLibA_10058	−3.49	TP53RK	HGLibA_58364	−2.48	RET	HGLibA_48160	−2.81
PKMYT1	HGLibA_44123	−3.41	PDPK1	HGLibA_43219	−2.47	PKMYT1	HGLibA_44123	−2.80
CDK11A	HGLibA_08726	−3.40	PRKAA1	HGLibA_45780	−2.43	MUSK	HGLibA_37865	−2.73
PDPK1	HGLibA_43219	−3.36	SCYL1	HGLibA_50372	−2.32	PHKG1	HGLibA_43699	−2.72
IKBKE	HGLibA_30211	−3.30	PKMYT1	HGLibA_44123	−2.31	PIK3R3	HGLibA_43942	−2.67
CSNK1D	HGLibA_11388	−3.28	PRKCD	HGLibA_45826	−2.29	DYRK2	HGLibA_14277	−2.62
CDK11B	HGLibA_08729	−3.21	TBK1	HGLibA_55890	−2.27	TBK1	HGLibA_55889	−2.52
CDK4	HGLibA_08771	−3.17	CDK12	HGLibA_08731	−2.25	PAK4	HGLibA_42338	−2.46
AURKA	HGLibA_03873	−3.14	CLK2	HGLibA_10058	−2.25	RIPK2	HGLibA_48607	−2.44
LTK	HGLibA_34900	−3.02	CDK2	HGLibA_08756	−2.22	PRKAA1	HGLibA_45780	−2.43

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ISSN: 1471-2164

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