Table 7 Summary of the sequencing methods, organisms, and mRNA extraction sources
From: Comparative transcriptomics enlarges the toolkit of known developmental genes in mollusks
Organism | Class | mRNA source | Sequencing |
|---|---|---|---|
Gymnomenia pellucida | Neomeniomorpha | 1/5 total RNA from developmental stages (i.e. freshly hatched larvae until metamorphosis) – 4/5 mRNA from adults. | Illumina |
Wirenia argentea | Neomeniomorpha | 1/7 total RNA from developmental stages (i.e. freshly hatched larvae until metamorphosis) – 6/7 mRNA from adults. | Illumina |
Scutopus ventrolineatus | Chaetodermomorpha | Total RNA from 2 adult individuals | Illumina |
Acanthochitona crinita | Polyplacophora | Early cleavage stages – gastrula – early, midstage, and late trochophore larvae – metamorphic competent and settled (post metamorphic) individuals | Illumina |
Idiosepius notoides | Cephalopoda | Central nervous system of 7 adult individuals | Illumina |
Idiosepius notoides | Cephalopoda | 2/3 total RNA from mixed developmental stages (i.e. stages collected after egg laying until the hatching stage) – 1/3 total RNA from adult central nervous system (brain), arm, and gonads tissue) | 454 |
Lottia cf. kogamogai | Gastropoda | 2/3 total RNA from mixed developmental stages (i.e. trochophore – veliger – pediveliger – metamorphic competent – first juvenile stages) – 1/3 total RNA from adult foot, and central nervous system (CNS) | 454 |
Nucula tumidula | Bivalvia | Early cleavage stages – gastrula – early, midstage, and late pericalymma larvae – metamorphic competent and settled (post metamorphic) individuals | Illumina |
Antalis entalis | Scaphopoda | Early cleavage stages – gastrula – early, midstage, and late trochophore larvae – metamorphic competent and settled (post metamorphic) individuals | Illumina |