Table 3 List of primers for C.cochliodes peroxidase & catalase genes
B | Primer description | Sequence in 5′ → 3′ direction | Tm [°C] | PCR prodct size [bp] |
|---|---|---|---|---|
hyBpox1 | CcochhyBpox1Fwd | CGAGAAAACAGATATTCTAGAAGCCA | 60.1 | 116 |
CcochhyBpox1Crev | TTCTACCGGCACCTAAATTGTT | 56.5 | ||
hyBpox2 | CcochhyBpox2aFWD | GTTCATTTAGCAGGAGGTCAGG | 60.3 | 119 |
CcochhyBpox2aREV | TGTCACTGCTCGAGTTAGCATT | 58.4 | ||
cyox1 | CcochCyox1bFWD | GCCTTCAAACTCCTCAACAAAG | 58.4 | 117 |
CcochCyox1bREV | GTAGCCGTCATGGAGGTTGTAT | 60.3 | ||
lds | CcochLDS3FWD | AACTTACACCATCTCCCGTGTC | 58.4 | 127 |
CcochLDS3REV | GTCGTACTGAGCGTCGTTGTAA | 60.3 | ||
dyPrx | CcochDyprxBfwd2 | AAAGGAATGTCGAACCAAAAGA | 54.7 | 135 |
CcochDyprxBrev1 | GCCGAGAGTAAAATCTGGAATG | 58.4 | ||
htp1 | CcochHtp1fwd2 | ATCTTCAACCAGACCATCTTCG | 58.4 | 114 |
CcochHtp1rev2 | GAACGACTTGGACTCGATCTG | 59.8 | ||
katA2 | CcochkatA2_IFWD | GAATCAACAAGACGCTTTGTGG | 63.5 | 202 |
CcochkatA2_IREV | TAGGTGGGTTAGCAAGTGAGAG | 63.3 | ||
katB1 | CcochkatB2_2REV | TAAACACAAAGTCCTGGTTCCC | 58.4 | 207 |
CcochkatB1_2REV | TGGAAAAGGCGCCGTAGTCG | 61.4 | ||
katB2 | CcochkatB2_1FWD | GGGGCGAGTTTGAGGTGACC | 63.5 | 198 |
CcochkatB2_2REV | TAAACACAAAGTCCTGGTTCCC | 58.4 |