Fig. 4

Elaboration of functional gene clusters in erythroid cells derived from adult and hiPSC progenitors in SEM-i. a PCA of genes differentially expressed in AB-erythroblasts (red symbols) or hiPSC-erythroblasts (blue symbols) during erythroid maturation in SEM-i. The gene set was any gene DE during maturation of erythroblasts from either source. The Euclidean distances relating to this PCA are available in Additional file 19: Table S8. b Expression profiles of globin genes in AB-erythroblasts and hiPSC-erythroblasts. Mean expression +/- standard error of the mean is plotted. c Expression profiles of genes encoding proteins with key roles in the regulation of erythroid development in the adult and hiPSC-derived settings. Mean expression +/- standard error of the mean is plotted. d Hierarchical clustering analysis by Euclidean distance of AB-erythroblasts and hiPSC-erythroblasts, clustered by gene only. The colour bar on the left hand side denotes clusters of co-regulated genes. e Gene expression profiles for c-KIT and NFIA in AB-erythroblasts and hiPSC-erythroblasts. Mean expression +/- standard error of the mean is plotted. Given these findings, we validated the microarray-based gene expression measurements of selected transcripts using quantitative RT-PCR (in Additional file 13: Figure S10B)