Fig. 3
Whole genome transcriptional analysis in Montepulciano (MP) and Sangiovese (SG) leaves subjected to water stress (WS). PCA and heat-map representation of the PCA loadings (first and last percentile) shows the significantly modulated genes in MP (a, c) and SG (b, d) vines under well-watered control (WW) and WS conditions. Expression was measured as the log2 intensity of each biological replicate. Each value was normalized on the median value of each row/gene and Euclidean’s correlation distance was used as the metric. e Venn diagram summarizing the differentially expressed genes retrieved by distinct approaches (see text). f, Enriched GO terms for the 1034 stress-modulated genes. The network graphs show BiNGO visualizations of the overrepresented GO terms. Categories in GoSlimPlants [66] were used to simplify this analysis. Colored nodes represent GO terms that are significantly overrepresented (p < 0.1). g Significant profiles (<5 % Bonferroni correction method) of the 1034 modulated genes during WS, from among 30 profiles subjected to STEM analysis, in MP (left column) and SG leaves (to the right of the yellow bar columns). In each frame, the number of genes shown in each profile is displayed to the bottom left whereas the number ID for each profile (from 1 to 30) is shown to the top left. Red curves represent individual profiles (i.e. profile of the fold change between WW and WS conditions) and the black line represents the profile to which they are most similar. Significant expression profiles are highlighted in color wherein the same background color represents a similar profile. Frames of the compared set profile (SG, to the right of the yellow bar) also show the comparison statistics. The correlation between MP and SG profiles is shown to the upper right as a number (1.00 indicates the same profile). The number of genes assigned to the MP profile that were also assigned to the SG profile is shown at the bottom left, followed by an indication of the p-value for the number of genes at the intersection. See Additional file 4 for the complete comparison profile table. The x-axis represents sampling points and the y-axis denotes log2 scale fold change in expression