Fig. 1

a The phylogeny of Dictyostelida. A maximum likelihood phylogeny of Amoebozoa with sequenced genomes based on a concatenated data set of 30 genes. Orthologs between all species were selected as in [27]. The tree was rooted with metazoa, plant, and fungi orthologs (not shown). Scale bar is in millions of years (mya), adjusted using dated splits of animals and plants (520 and 670 mya, respectively). b The morphological stages of sampling and a flowgram for the three different analysis methods of the RNAseq data. Morphological stages are purely schematic similar to DD stages, the fruiting body morphology differs between species