Fig. 2

a Percentage of yeast and mycelium in Ophiostoma novo-ulmi at 0, 2, 4, 6, 10 and 27 h of incubation in conditions inducing the yeast-to-hypha transition. This was achieved by transferring yeast cells grown in shake liquid minimal medium to fresh liquid complete growth medium (OCM) without agitation. Values are means and standard deviations for three replicates. Black: yeast percentage; Red: Hyphae percentage. b Cell size in μm at each of the 6 time points for the three replicates. c Density of cell size (gray panels) and density of cell granularity or internal complexity (white panels) at each of 5 time points measured by flow cytometry. FSC: Forward Scatter, cell size. FSC: Side Scatter, granularity. OMM: minimum medium under agitation, no transition. OCM: Ophiostoma complete medium in still conditions, switch from yeast to mycelium. d Scanning-electron microscopy pictures taken at each time point. Scale is indicated on the pictures. OY: ovoid yeast cells; SY: spherical yeast cells; M: extracellular matrix. Red arrows indicate septa