Fig. 1
From: DeSigN: connecting gene expression with therapeutics for drug repurposing and development
Workflow of DeSigN. a A reference database of cell lines that are sensitive and resistant to drugs available in the GDSC database was created. Version 1.0 contains 140 drugs with their unique ranked-based gene signatures. b Differential expressed gene signatures are generated from differential expression analysis of cell lines from two distinct experimental conditions, e.g. cell line gene expression data from tumour samples versus normal samples. The up and down-regulated genes (log2 fold change > 1 and p-value < 0.01) thus selected will be used to query the DeSigN database. c A ranked-based list of inhibitors is generated, with Connectivity Score between 1 (maximal efficacy) and −1 (minimal efficacy). This allows users to prioritize the testing of these candidates