Table 1 Genes used in “BioMolChem” chip that were analyzed in the Stratagene Mx3005P qPCR system, classified according to functions and pathways
Defense-related genes | Names | N° accession GeneBank | Forward primer (5′-3′) | Reverse primer (5′-3′) | |
|---|---|---|---|---|---|
Reference gene | γ-chain of Elongation Factor 1 | EF1γ | AF176496 | GAAGGTTGACCTCTCGGATG | AGAGCCTCTCCCTCAAAAGG |
PR proteins | PR1 Unknown function | PR1 | AJ536326 | CCCAGAACTCTCCACAGGAC | GCAGCTACAGTGTCGTTCCA |
Beta-1,3-glucanase (PR2) | GLU | AF239617 | GGGGAGATGTGAGGGGTTAT | TGCAGTGAACAAAGCGTAGG | |
Endochitinase (Chitinase IV - PR3) | CHIT4a | U97521.1 | TATCCATGTGTCTCCGGTCA | TGAATCCAATGCTGTTTCCA | |
proteinase inhibitor (PR6) | PIN | XM_002284418 | ACGAAAACGGCATCGTAATC | TCTTACTGGGGCACCATTTC | |
Chitinase III - (PR8) | CHIT3 | Z68123 | AATGATGCCCAAAACGTAGC | ATAAGGCTCGAGCAAGGTCA | |
PR protein - class 10 (PR10) | PR10 | AJ291705 | GCTCAAAGTGGTGGCTTCTC | CTCTACATCGCCCTTGGTGT | |
Polygalacturonase Inhibiting Protein | PGIP | XM_002263487.1 | GAGCGATGCCACCCCAGTGA | CCGTTGAGTCGGACGCTCGAC | |
Phenylpropanoid pathways | Phenylalanine ammonia lyase | PAL | X75967 | ACAACAATGGACTGCCATCA | CACTTTCGACATGGTTGGTG |
Stilbene synthase | STS | X76892.1 | ATCGAAGATCACCCACCTTG | CTTAGCGGTTCGAAGGACAG | |
Chalcone Synthase | CHS | X75969.1 | CCAACAATGGTGTCAGTTGC | CTCGGTCATGTGCTCACTGT | |
Chalcone Isomerase | CHI | X75963 | AGAAGCCAAAGCCATTGAGA | CCAAGGGGAGAATGAGTGAA | |
Anthocyanidine synthase | LDOX | X75966 | TGGTGGGATGGAAGAGCTAC | CCCACTTGCCCTCATAGAAA | |
Flavanone-3-hydroxylase | F3H | X75965.1 | TGACTCGCTCTCTTCAAGCA | CACCTTGGGACGTTCATCTT | |
Indole pathways | Antranilate Synthase | ANTS | XM 002281597 | AAAAATCCAAGAGGGGTGCT | AAGCTTCTCCGATGCACTGT |
Chorismate mutase | CHORM | FJ604854 | TCATTGAGAGGGCCAAATTC | AGGAGGCAGAAAAAGCATCA | |
Chorismate Synthase | CHORS | FJ604855 | GCCTTCACATGCAGATGCTA | CTGCAACTCTCCCAATGGTT | |
Redox status | Glutathione S-transferase | GST1 | AY156048.1 | GGGATCTCAAAGGCAAAACA | AAAAGGGCTTGCGGAGTAAT |
Lipoxygenases | Lipoxygenase-9 | LOX9 | AY159556 | GACAAGAAGGACGAGCCTTG | CATAAGGGTACTGCCCGAAA |
Signaling | 1-aminocyclopropane, 1-carboxylic oxidase | ACC | AF424611 | GAAGGCCTTTTACGGGTCTC | CCAGCATCAGTGTGTGCTCT |
Cell Wall Reinforcement | Glycosyl transferase | CAGT | XM02273320.1 | TCGGAAGGGAATGCAATAAG | TGTAGGAGGAACCACCCTTG |
Callose Synthase | CALS | AJ430780.1 | TGGAAATGCAATTCAAACGA | CGAATGCCATGTCTGTATGG | |
Lignin-forming peroxidase | PER | XM_002274762.1 | TAAGCGCCACAAGAACACTG | GGACCTCCTTGTTGAGTCCA |