Fig. 2

Illustration of how barcode level read evidence is evaluated for a hypothetical barcode with 8 read pairs, assuming all reads have either T (reference) or A (alternative) at the specific locus. The x-axis is the number of read pairs with the alternative allele A, ranging from 0 to 8. The y-axis is − log10(1−P(A|BC)), the amount of read evidence for A assign to the barcode. Overall, the amount of read evidence for A is close to 0 when less than half of the reads are A, and then gradually increases