Fig. 1From: Enumerateblood – an R package to estimate the cellular composition of whole blood from Affymetrix Gene ST gene expression profilesSchematic representation of the experiment. Cell proportions were estimated from DNA methylation profiles for 172 samples (1; COPD patients). These DNA methylation-derived cell proportions were used as a ‘silver’ standard in the absence of the ground truth. This ‘silver standard’ dataset was used to train a multi-response Gaussian model using the gene expression data (2). Out-of-sample performance was evaluated using a repeated (20x) 10-fold cross-validation (3) and in two independent sets of samples, in different clinical indications: granulocyte, monocyte, and lymphocyte performance was evaluated in a large set of samples (4; 197 samples, heart failure), while monocyte, B, and T cell prediction performance was evaluated in a smaller set (4; 28 samples, asthma)Back to article page