Fig. 4

Insertion of the genomic NZO fragment containing the Ifi200 cluster into the B6 strain induces obesity. Body weight (a) and fat mass (b) development of Nob3.14 ^B/B (n = 9) and Nob3.14 ^N/N (n = 9) female mice kept on HFD. c Gonadal white adipose tissue (gonWAT) mass of Nob3.14 female mice (n = 6). d Histological analysis of gonWAT of Nob3.14 ^B/B and Nob3.14 ^N/N mice. Scale bar, 50 μm. Western blot analysis indicated an increased expression of the adipogenic marker PPARy (e) and the lipolytic enzyme pHSL (f) in gonWAT of congenic mice carrying the Nob3.14 ^N/N locus in comparison to controls (Nob3.14 ^B/B). Data are presented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001 by t-test