Fig. 3
From: 2.7 million samples genotyped for HLA by next generation sequencing: lessons learned
Reads passing filter vs. cluster density on Illumina MiSeq and HiSeq instruments. Each data point represents a run (flowcell). Shaded areas denote supported ranges of cluster densities and expected output for different chemistries/kits as specified by Illumina. The colour gradient indicates the total percentage of bases reaching a quality score of 30 or higher per run. Trend lines are generated by generalised additive model fits using a cubic penalised regression spline. M = millions