Fig. 5
From: 2.7 million samples genotyped for HLA by next generation sequencing: lessons learned
Dependency of primer dimer rate from initial DNA concentration and workflow. The two workflows differ both in their reaction volumes and amplification strategies (Fluidigm: single PCR, 4 primers; 384 PCR: 2 PCRs, 2 primers each). Solid lines depict generalised additive model fits using a cubic penalised regression spline. The shaded bands around the regression lines indicate the pointwise 95% confidence intervals on the fitted values