Table 1 qRT-PCR primer and size of the amplification products of the target and housekeeping genes
From: Screening and evaluating of long noncoding RNAs in the puberty of goats
Gene | Forward primer, 5’-3’ | Reverse primer, 5’-3’ | Product size, (bp) |
|---|---|---|---|
XLOC_957527 | ACACGACCAGAACATCAG | AATCACAGGAGAAGAGTAGG | 162 |
XLOC_910648 | TGCCATCCAGCCATCTCATC | CACACACCACAGTTCCTTTACC | 175 |
XLOC_1101518 | CTCCTGGGCTACCGAATGT | GCGGCTGTGAACTAAATGG | 172 |
XLOC_1276445 | TCGCTCCGTCTTCACCTAC | CGTTGCTCCATCACCCTTG | 100 |
XLOC_2056339 | CCTGTTGTTGGAATCACTC | CTCTTATGCCTCGGATGG | 184 |
XLOC_960044 | CAAGGAGTCGCACGCTAC | CTCTTACGCCTCTGAATCGG | 128 |
XLOC_032671 | TGATGCCAAGAGGTAGCC | TTATACAGACAGTGAAAGAGAGG | 180 |
XLOC_688924 | ATCTCCACTCTACAAACCTATACC | ACTCTCAAAGGGAAGCCAATG | 142 |
Novel_000476 | TTACCTACTACCATCCTTCAC | ATCAGCAGAACCAGAACC | 178 |
Novel_000453 | GAAGTGTCGTCTGGAGATTACC | TGTTGAGTGAGTCCTGTATTACC | 181 |
CD38 | CTACTGCCTTCTTCTGTG | TTCTGCTTCTGGAATACG | 185 |
GnRH1 | CTAATCCTGCTGACTTTCTGTG | ACCTCTTTGGCTATCTCTTGG | 127 |
β-actin | CGTGACATCAAGGAGAAG | GAAGGAAGGCTGGAAGAG | 171 |