Unity in defence: honeybee workers exhibit conserved molecular responses to diverse pathogens

BMC Genomics

Table 2 List of the 19 transcriptome datasets

#	Parasite	Cat.	Age (days)	Days p.i.	Tissue	Technology	Reference
1	N. ceranae	N	15	13	Brain	RNA-seq	[24]
2	N. ceranae	N	10	10	Brain	RNA-seq	[25]
3	N. ceranae	N	14	7	Midgut	Tiling array	[26]
4	N. ceranae	N	13	12	Abdomen	Microarray	[74]
5	N. apis	N	3	1	Midgut	Microarray	[27]
6	N. apis	N	8	2	Midgut	Microarray	[27]
7	N. apis	N	2	2	Fat body	Microarray	[27]
8	N. apis	N	3	7	Fat body	Microarray	[27]
9	N. apis and N. ceranae ^a	N	15	14	Fat body	Microarray	[27]
10	N. ceranae and BQCV ^a	N/V	15	13	Brain	RNA-seq	[24]
11	N. ceranae and DWV ^a	N/V	13	12	Abdomen	Microarray	[74]
12	SINV-GFP ^b	V	4	3	Whole bee	Microarray	[29]
13	DWV	V	pupae	3	Brain	Microarray	[75]
14	DWV	V	13	12	Abdomen	Microarray	[74]
15	BQCV	V	15	13	Brain	RNA-seq	[24]
16	IAPV	V	1	1	Fat body	RNA-seq	[28]
17	V. destructor ^c	M	10	-	Brain	RNA-seq	[25]
18	V. destructor (N = 1 mite) ^d	M	1	12	Whole bee	RNA-seq	[76]
19	V. destructor (N = 3 mites) ^e	M	1	12	Whole bee	RNA-seq	[76]

All datasets were generated from worker honey bees experimentally infected by Varroa mites, RNA viruses and/or Nosema spp., and for which gene expression was compared with uninfected control samples. Note that Varroa parasitism was also associated with high viral titers and therefore represented a ‘Varroa plus virus’ treatment. BQCV black queen cell virus, IAPV Israeli acute paralysis virus, DWV deformed wing virus. Categories (Cat.) are N for Nosema, N/V for Nosema and virus co-infection, V for virus alone and M for Varroa mite (‘Varroa plus virus’), as used across this study. Age and Days p.i. gives the age (i.e. days post-eclosion) and the number of days post infection when bees were collected for transcriptome analysis
^a Studies where honey bees were co-infected with two pathogens
^b This study used the model Sindbis virus expressing enhanced green fluorescent protein (SINV-GFP)
^c Transcripts from DWV (4 to 15 × 10⁵ tags) and Varroa destructor virus (21 to 25 × 10⁶ tags) present in brain transcriptomes from Varroa infested bees
^d Average proportion of reads attributed to DWV = 37.6% (±14.8 sem)
^e Average proportion of reads attributed to DWV = 47.7% (±17.7 sem)

ISSN: 1471-2164