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Table 2 SNP statistics reported by GATK and thresholds used for filtering

From: Positional bias in variant calls against draft reference assemblies

Abbreviation

Rel

Threshold

Full name

QD

<

2.0

Quality by Depth

MQ

<

40.0

Root mean square of Mapping Quality

MQRankSum

<

−12.5

Mapping Quality Rank Sum test

FS

>

60.0

Fisher’s exact test for Strand bias

SOR

>

4.0

Strand bias Odds Ratio

ReadPosRankSum

<

−8.0

Read Position Rank Sum test

DP

>

200.0

Depth of Coverage

GQ

<

20.0

Genotype Quality

  1. SNPs with statistics above or below (Rel) the corresponding threshold were removed from consideration. For detailed description of these statistics and justification for the threshold selection, see Van der Auwera et al. [24] and GATK documentation at https://www.broadinstitute.org/gatk/