Skip to main content

Table 4 Validation of microarray data for selected differentially expressed transcripts by qPCR

From: Analysis of hepatic transcript profile and plasma lipid profile in early lactating dairy cows fed grape seed and grape marc meal extract

 

Mean FC

P-value

Gene symbol

Microarray

qPCR

Microarray

qPCR

STMN1

2.57

1.37

0.015

0.269

ECT2

2.53

1.45

0.023

0.246

CENPA

2.49

1.16

0.006

0.514

CENPF

2.45

1.07

0.017

0.788

CKAP2

2.39

1.20

0.023

0.557

PRR11

2.32

1.93

0.036

0.181

KIF20A

2.21

1.66

0.017

0.088

BUB1B

2.20

1.22

0.004

0.528

RRM2

1.95

1.56

0.046

0.263

ESCO2

1.93

–1.02

0.032

0.905

SPC25

1.88

–1.19

0.034

0.207

CCNA2

1.87

–1.07

0.030

0.762

HMMR

1.87

–1.34

0.018

0.100

TUBB

1.85

1.38

0.013

0.040

GLCE

–1.66

–1.2

0.036

0.195

MANF

–1.59

–1.23

0.014

0.235

SAA4

–1.54

–1.31

0.043

0.057

SOCS3

–1.45

–1.14

0.042

0.522

PHLDA1

–1.43

–1.34

0.012

0.006

HYOU1

–1.39

–1.33

0.018

0.081

DNAJB11

–1.37

–1.23

0.026

0.170

BAG3

–1.37

1.07

0.032

0.674

UAP1

–1.34

1.05

0.005

0.674

CCNL1

–1.33

–1.26

0.009

0.067

CXCL14

–1.31

–1.16

0.036

0.459

  1. The microarray FC was calculated from the signal log ratios as follows: 2Signal log ratio if signal log ratio ≥ 0 and (−1) × 2–(Signal log ratio) if signal log ratio < 0. Signal log ratios were calculated from n = 6 microarrays per group. The qPCR FC was calculated analogously from normalized 2-ΔCt ratios. Normalized 2-ΔCt expression was calculated from n = 14 samples per group
Back to article page

BMC Genomics

ISSN: 1471-2164

Contact us