High-throughput sequencing of pituitary and hypothalamic microRNA transcriptome associated with high rate of egg production

Table 2 Evaluation of the expression profile variation between RNA-Seq and RT-qPCR for the selected miRNAs

Tissue	miRNA	Fold change (HP/LP)		P-value
Tissue	miRNA	RNA-Seq	RT-qPCR	RNA-Seq	RT-qPCR
pituitary	gga-miR-1744-3p	0.32	0.66	0.0296	0.0279
	gga-miR-122-5p	0.26	0.23	0.0039	0.0095
	gga-miR-1434	0.42	0.28	0.0464	0.0229
	gga-miR-99a-5p	0.87	0.78	0.6680	0.1060
	gga-miR-26a-5p	0.84	0.63	0.5370	0.2780
	gga-miR-34b-3p	3.34	3.53	0.0377	0.0477
	gga-miR-34c-3p	3.86	3.39	0.0362	0.0401
	gga-miR-1684a-3p	30.57	31.12	0.0004	0.0010
hypothalamus	gga-miR-1744-3p	0.15	0.23	0.0021	0.0064
	gga-miR-34b-3p	0.16	0.22	0.0023	0.0012
	gga-miR-34c-3p	0.18	0.14	0.0004	0.0062
	gga-miR-99a-5p	1.34	1.31	0.3620	0.0685
	gga-miR-26a-5p	1.19	0.69	0.5430	0.2510
	gga-miR-122-5p	7.41	6.63	0.0021	0.0005
	gga-miR-1434	4.99	4.35	0.0018	0.0211
	gga-miR-1684a-3p	24.47	26.81	0.0037	0.0006

ISSN: 1471-2164