Fig. 2

Linear regression fitted for Log₂ Fold Change (FC) of selected genes determined via Biomark assay and RNA-seq technology. Different contrasts were marked in different colors. Different groups in the comparisons were labeled as Line_Treatment (RS: Ross, IL: Illinois, HS: heat stress, TN: thermoneutral). R-square and Pearson correlation coefficient are labeled as “R²” and “r”. Log₂FC in Biomark assay equals -ΔΔC_t for each comparison. Average C_t value for each group was the means of samples in that group. Average expression of three housekeeping genes including GAPDH, H6PD and RPS13 were used for normalization of C_t values