Fig. 4

Relative Expression of NrrF Regulated Fe dependent Nrs sRNAs. The relative concentration of Nrs sRNAs was assessed by real time PCR in WT strain FA 1090 and nrrf mutant strain LJ001 in Fe restricted growth conditions. Values reported are means from three separate growth curves ± SEM at 1,2,3,4 and 5 h. Relative concentration significantly increased in the mutant strain compared to WT for Nrs sRNAs, with increased levels at late log and as early as 2 h a-e). *Difference in relative concentration between FA 1090 and nrrf mutant were significant at (P < 0.05)