Fig. 8

CYP6DE3 enzyme assays. GC chromatograms of pentane:ether (1:1) extracts from recombinant CYP6DE3 incubated for one hour in the presence of 6 mM a (+)-α-pinene, b 3-carene or c (+)-limonene, 3 mM NADPH and recombinant house fly CPR or with CPR alone (negative control). Below are mass spectra for selected retention peaks showing a production of 3-Oxatricyclo [4.1.1.0(2,4)]octane at 10.88 min, trans-verbenol at 12.15 min and two unknown products at 15.42 and 15.65 min for (+)-α-pinene, b two unknown products at 14.78 and 15.92 min for 3-carene and c to unknown products 16.00 and 16.12 for (+)-limonene. The 15.65 min product for (+)-α-pinene and the 14.78 min product for 3-carene have m/z peaks at 168 indicating a possible double oxidation of (+)-α-pinene and 3-carene