Table 1 Primers used for quantitative real-time PCR
Gene | Gene Bank ID | Primers | Primer sequence (5’-3’) | Amplicon Size | References |
|---|---|---|---|---|---|
HLA-DQA1 | BT020994.1 | Forward | CCTTGTGGGTATCGTGGTGG | 150 bp | This study |
Reverse | CGTCTAGCACGTCCACTCTT | ||||
PI3 | XM_005214890.3 | Forward | TGACTGGGCAAGGGGAGCCG | 112bp | This study |
Reverse | GGGGCCACCCCAAAGAAGCC | ||||
HSPB8 | BT020640.1 | Forward | CAGTCTTGGCCCTTCCTTGT | 176bp | This study |
Reverse | TCAATTGCGCCATCTTGCAG | ||||
PRSS53 | XM_015469379.1 | Forward | TGCACAGCTAACATGAGCCA | 148 bp | This study |
Reverse | CCAGAGCCCTTTGCACTGTA | ||||
DSG1 | NM_174045.1 | Forward | ATCCAACTGACTTGCTCGCT | 278 bp | This study |
Reverse | ACCACCACCAGTTGTGTAGC | ||||
BAG3 | BC133574.1 | Forward | ACGCAGTAACTTGGGTGGAG | 148 bp | This study |
Reverse | ACAGAGACGGCTCCAAAACA | ||||
CYR61 | NM_001034340.2 | Forward | GATGCAACTACAACTGCCCG | 116 bp | This study |
Reverse | CCACCCTTATGCTGGAAGCC | ||||
IGFBP3 | NM_174556.1 | Forward | TTAATGCCTGCACATCCCGA | 259 bp | This study |
Reverse | CCCTATGGGGCTTCAGCAAA | ||||
GAPDH | BC102589 | Forward | TGGAAAGGCCATCACCATCT | 60 bp | [31] |
Reverse | CCCACTTGA TGTTGGCAG | ||||
β-actin | NM-173979 | Forward | GCCATGAAGCTGAAGATGAC | 229 bp | [29] |
Reverse | CCTTCTGCAGCTCAGATATG |