Terminus predicted by NGS data | Terminus identified by primer walking sequencing | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
Name | Sequencer | Contig size | a5′ ter. | b3′ ter. | 5′ ter. | cdistance | 5′ terminus flanking sequence | 3′ ter. | distance | 3′ terminus flanking sequence | length of DTR |
SBP8a | Roche/454 | 158794 | 111794 | 114616 | 111796 | +2 | 5′-AGGTAGAACG | 114616 | 0 | AGAAAAACCT-3′ | 2821 |
dI13 | PGM | 157905 | 111610 | 114359 | 111537 | −73 | 5′- AGGTAGAACG | 114359 | 0 | AGAAAAACCT-3′ | 2823 |
dI22 | PGM | 157889 | 106421 | 109166 | 106348 | −73 | 5′- AGGTAGAACG | 109169 | +3 | AGAAAAACCT-3′ | 2822 |
Q8 | PGM | 158180 | 156549 | g5025 | 156549 | 0 | 5′- AGGTTTTGTG | 5099 | +74 | GGGTCTACCC-3′ | 6731 |
eQ10 | PGM | 158174 | 86402 | g93056 | 86402 | 0 | 5′- AGGTTTTGTG | 93130 | +74 | GGGTCTACCC-3′ | 6729 |
Q11 | MiSeq | 26005 | N/A | N/A | h-9 | N/A | 5′ AAAATGTAAA | h + 43 | N/A | ATATACATTT-3′ | N/A |
fI46 | MiSeq | 24896 | N/A | N/A | h-740 | N/A | 5′ AAAATGTAAA | h + 421 | N/A | ATATACATTT-3′ | N/A |