Fig. 5

Quantitative northern blot analysis of miRNA-like candidates (a) miRCat-2 and (b) Folded-2. Individual lanes were loaded with 4 μg sRNA which were blotted and onto membranes and hybridised at 37 °C overnight with. P³² labelled oligonucleotides designed to detect miRNA-like candidate species. A. fumigatus U1-1 small nuclear RNA was used as a loading control throughout and the miRNA bands were normalised using the integrity of the U1-1 bands. Relative expression levels were plotted using the GraphPad Prism 6.0 programme and P values were estimated using unpaired t-test with Welch’s correction. Error bars were calculated using the standard error values. P values less than 0.05 were accepted as statistically significantly different and are shown with an asterisk (** indicates P ≤ 0.01; n = 3)