Fig. 5From: Differentially expressed genes from RNA-Seq and functional enrichment results are affected by the choice of single-end versus paired-end reads and stranded versus non-stranded protocolsDifferentially expressed genes (DEGs) identified in each of the four experiments. Venn diagrams of the DEGs comparing those derived from the PE data (orange) with those derived from the SE data (purple) and the NS data (green). Differentially expressed genes were identified using edgeR (a-d) and voom (limma) (e-h) with an FDR cut-off of 0.05. a, e Experiment 1, (b, f) Experiment 2, (c, g) Experiment 3, (d, h) Experiment 4Back to article page