Fig. 1

Pipeline for NIRVS identification. The currently available 22 mosquito genomes and the genome of Drosophila melanogaster were probed bioinformatically using tblastx and 425 viral species (424 non-retroviral RNA viruses and 1 DNA arbovirus). Tested insect and viral RNA genomes are shown in the context of their phylogeny [2, 38]. Identified blast hits were parsed based on gene ontology and the presence of partial or complete viral ORFs. In Ae. albopictus, bioinformatic analyses was extended to whole-genome sequencing data from 16 individual mosquitoes of the Foshan strain. This stringent pipeline led to the characterization of 242 loci with NIRVS. Viral families for which NIRVS were characterized are shown in red