Development of a high-density linkage map and mapping of the three-pistil gene (Pis1) in wheat using GBS markers

Table 3 The positions of the candidate genes nearest to Pis1

No.	Start position	End position	BLAST matching accession no.	Annotation	Sequence identity (%)	E value
1	139,244,852	139,245,784	EMT28401	N/A
2	139,342,698	139,344,779	ACT22500	plastid glutamine synthetase 2 [Triticum aestivum]	98%	9e-66
3	139,348,585	139,351,702	EMS55336	DNA topoisomerase 1 [Triticum urartu]	80%	0
4	139,364,799	139,366,357	EMS51822	Transmembrane 9 superfamily member 4 [Triticum urartu]	97%	0
5	139,369,519	139,370,195	EMT08392	Tropinone reductase-like protein [Aegilops tauschii]	88%	4e-53
6	139,380,317	139,381,181	EMT03631	N/A
7	139,385,118	139,386,329	EMS51820	N/A
8	139,393,899	139,394,513	EMT03801	Transmembrane 9 superfamily member 2 [Aegilops tauschii]	98%	1e-93
9	139,406,363	139,408,447	EMT09611	U3 small nucleolar ribonucleoprotein IMP3 [Aegilops tauschii]	99%	2e-130
10	139,416,283	139,418,780	NP_001150358	N/A

ISSN: 1471-2164