The genome of the biting midge Culicoides sonorensis and gene expression analyses of vector competence for bluetongue virus

Table 1 A comparison of genome characteristics of the C. sonorensis genome with other selected Diptera species

Parameter	Culicoides sonorensis	Aedes aegypti L3	Aedes aegypti L5^a	Anopheles gambiae AGAM P4^a	Drosophila melanogaster BDGP6^a	Belgica antarctica GCA_000775305.1
Genome size (Mb)	189	1384.1	1278.7	273.1	143.7	89.7
Number of contigs	15,810	36,205	2539	16,825	2442	22,492
Contig N50	30,774	82,500	11,758,062	85,555	19,488,218	13,551
Contig N90	5567	15.283	74,389	5600	666,663	1865
Number of scaffolds	7974	4757	2310	8	1870	4997
Scaffold N50	89,077	1,547,048	409,777,670	49,364,325	25,286,936	98,164
Scaffold N90	14,215	324,062	83,687	2684	23,513,712	18,418
Ns (%)	2.8	5.3	0.0	7.6	0.8	0.9
Maximum contig length	552,397	685,587	71,953,859	808,130	27,905,041	131,402
Repeats + low complexity regions	29.7%	73%	78%	25%	23%	4%
GC content	28.4%	38.3%	38.2%	44.3%	42.0%	38.9%
GC exons	34.3%	46.8%	46.2%	54.6%	49.1%	47.1%
Protein-coding genes	15,612	15,796	14,626	13,075	13,918	13,510
Mean protein-coding gene length	5040 bp	18,126 bp	46,920 bp	6442 bp	6960 bp	2555 bp
Mean exon length (protein coding genes)	480 bp	460 bp	494 bp	438 bp	538 bp	325 bp
Mean intron length (protein-coding genes)	632 bp	23,265 bp	10,456 bp	1008 bp	1147 bp	213 bp
Mean introns per transcript (in protein-coding genes)	6.7	5.1	7.9	5.5	6.9	5.3
Maximum splice isoforms/protein-coding gene	19	20	50	20	75	n.a.

^ain the case of these species, the number of scaffolds shown correspond to the chromosomal arms plus minor scaffolds that may have been mapped but not assembled

ISSN: 1471-2164