Table 1 Main characteristics of ESBL-EC (A, n = 29) and non-ESBL-EC (B, n = 4) associated with qnrB from the WGS collection of the NRL-AR. Besides basic metadata (year of sampling and source), information on phenotypic resistance profiles, in silico-based prediction of acquired resistance determinants and chromosomal sequence alterations associated with (fluoro) quinolone resistance development as well as the MLST type is given
From: Characterization of qnrB-carrying plasmids from ESBL- and non-ESBL-producing Escherichia coli
Isolate | Year of sampling | Matrix (source) | Resistance profilea | Acquired resistance determinantsb | Chromosomal alterations associated with (fluoro)quinolone resistance | multilocus seuqence type (MLST) |
|---|---|---|---|---|---|---|
13-AB00983 | 2013 | Poultry, meat | AMP, CIP, COL, FOT, STR, TAZ | acrF, ant(3'')-Ia, blaEC, blaSHV-12, emrD, lnu(F), mcr-1.1, mdtM, qnrB19 | n.d | 753 |
14-AB00641 | 2014 | Poultry, meat | AMP, CIP, COL, FOT, TAZ | acrF, ant(3'')-Ia, blaEC, blaSHV-12, emrD, lnu(F), mcr-1.1, mdtM, qnrB19 | n.d | 48 |
16-AB00831 | 2016 | Poultry, meat | AMP, CIP, COL, NAL, TET | acrF, blaEC, blaTEM-1, emrD, mcr-1.1, mdtM, qnrB19, tet(A) | GyrA (S83L), ParC (S80I), ParE (S458A) | 1196 |
16-AB01284 | 2016 | Poultry, feces | AMP, CHL, CIP, COL, GEN, SMX, TET | aac(3)-IId, aadA1, aadA2, acrF, blaEC, blaTEM-1, cmlA1, emrD, mcr-1.1, mdtM, qnrB19, sul3, tet(A) | n.d | 58 |
16-AB01309 | 2016 | Poultry, feces | AMP, CHL, CIP, COL, FOT, NAL, SMX, TAZ, TET, TMP | aac(6')-Ib-cr5, aadA1, acrF, arr-3, blaCTX-M-65, blaEC, blaOXA-1, blaTEM-135, catB3, cmlA1, dfrA15, emrD, floR, mcr-1.1, mdtM, qnrB19, qnrS1, qnrS2, sul3, tet(A) | GyrA (S83L), ParC(S80I) | 2179 |
16-AB02042 | 2016 | Poultry, meat | AMP, CHL, CIP, COL, GEN, SMX, TET | aac(3)-IIe, aadA1, aadA2, acrF, aph(3'')-Ib, aph(3')-Ia, aph(6)-Id, blaEC, blaTEM-135, catA1, cmlA1, emrD, mcr-1.1, mdtM, qnrB19, sul3, tet(A) | n.d | 155 |
16-AB03538 | 2016 | Poultry, feces | AMP, CHL, CIP, COL, SMX, TET | aadA1, aadA2, acrF, aph(3')-Ia, blaEC, blaTEM-135, cmlA1, emrD, mcr-1.1, mdtM, qnrB19, sul3, tet(A) | n.d | 48 |
17-AB00706 | 2017 | Pig, feces | AMP, CIP, FOT, NAL, SMX, TAZ, TET, TMP | aadA5, acrF, aph(3'')-Ib, aph(6)-Id, blaEC, blaTEM-1, catB3, dfrA1, emrD, mdtM, qnrB19, sul1, sul2, tet(A) | GyrA (S83L, D87N) | 88 |
17-AB01697 | 2017 | Cattle, feces | AMP, CIP, FOT, SMX, TAZ, TMP | acrF, blaCTX-M-1, blaEC, dfrA25, emrD, mdtM, qnrB2, sul1 | n.d | 657 |
17-AB02713 | 2017 | Cattle, feces | AMP, CIP, FOT, SMX, TAZ, TET, TMP | aac(6')-Ib-cr5, aadA1, acrF, aph(3'')-Ib, aph(6)-Id, blaCTX-M-15, blaEC, blaOXA-1, blaTEM-1, catA1, catB3, dfrA1, dfrA14, emrD, mdtM, mef(C), mph(G), qnrB1, sul1, sul2, tet(A) | n.d | 398 |
17-AB02827 | 2017 | Pig, feces | AMP, CHL, CIP, FOT, GEN, NAL, SMX, TAZ, TET, TMP | aac(3)-IIe, aac(6')-Ib-cr5, aadA1, acrF, aph(3'')-Ib, aph(6)-Id, blaCTX-M-15, blaEC, blaOXA-1, blaTEM-1, catA1, catB3, dfrA14, emrD, mdtM, qnrB1, sul2, tet(A) | ParC (A56T, S80I) | 744 |
18-AB00078 | 2017 | Cattle, feced | AMP, CHL, CIP, FOT, SMX, TAZ, TET, TMP | aac(6')-Ib-cr5, aadA1, acrF, aph(3'')-Ib, aph(6)-Id, blaCTX-M-15, blaEC, blaOXA-1, blaTEM-1, catA1, catB3, dfrA14, emrD, floR, mdtM, qnrB1, sul2, tet(A) | n.d | 154 |
20-AB00274 | 2020 | Poultry, meat | AMP, CIP, FOT, TAZ, TET | acrF, aph(3')-Ia, blaCTX-M-55, blaEC, emrD, mdtM, qnrB19, tet(A) | n.d | 1011 |
20-AB00375 | 2020 | Poultry, feces | AMP, CIP, FOT, NAL, TAZ, TET | acrF, blaEC, blaSHV-12, blaTEM-1, emrD, mdtM, qnrB19, qnrS1, tet(A) | n.d | 1626 |
20-AB00564 | 2020 | Poultry, feces | AMP, CIP, FOT, TAZ | acrF, blaEC, blaSHV-12, emrD, mdtM, qnrB19 | n.d | 155 |
20-AB00569 | 2020 | Poultry, feces | AMP, CIP, FOT, TAZ | aadA2, acrF, blaEC, blaTEM-52, emrD, lnu(F), mdtM, qnrB19 | n.d | 192 |
20-AB00611 | 2020 | Poultry, feces | AMP, CIP, FOT, NAL, TAZ | acrF, blaCMY-2, blaEC, blaTEM-1, emrD, qnrB19 | GyrA (S83L), ParC (E84K) | 131 |
20-AB00922 | 2020 | Poultry, feces | AMP, CIP, FOT, NAL, TAZ | acrF, blaCMY-2, blaEC, emrD, qnrB19 | GyrA (S83L) | 131 |
20-AB01255 | 2020 | Poultry, feces | AMP, CIP, FOT, TAZ | acrF, blaEC, blaSHV-12, emrD, mdtM, qnrB19 | n.d | 1406 |
20-AB01339 | 2020 | Poultry, feces | AMP, CIP, FOT, TAZ | aadA22, acrF, blaEC, blaSHV-12, emrD, lnu(F), mdtM, qnrB19, qnrS1 | n.d | 162 |
20-AB01569 | 2020 | Poultry,feces | AMP, CHL, CIP, FOT, NAL, SMX, TAZ, TET, TMP | aadA1, acrF, aph(3'')-Ib, aph(6)-Id, blaCTX-M-27, blaEC, dfrA1, emrD, floR, mdtM, qnrB19, sul1, sul2, tet(A), tet(B) | GyrA (D87N, S83L), ParC (S80I) | 533 |
20-AB01574 | 2020 | Poultry, feces | AMP, CHL, CIP, FOT, SMX, TAZ, TET, TMP | aac(3)-IIe, aac(6')-Ib-cr5, aadA1, acrF, aph(3'')-Ib, aph(6)-Id, blaCTX-M-15, blaEC, blaOXA-1, blaTEM-1, catA1, catB3, dfrA14, emrD, mdtM, qnrB1, sul2, tet(A) | n.d | 3058 |
20-AB01775 | 2020 | Poultry, feces | AMP, CIP, COL, FOT, TAZ | acrF, blaEC, blaTEM-52, emrD, mdtM, qnrB19 | n.d | 226 |
20-MO00017 | 2018 | Pig, feces | AMP, CIP, FOT, GEN, SMX, TAZ, TET | aac(3)-IIe, aadA1, acrF, aph(3')-Ia, blaCTX-M-55, blaEC, emrD, mdtM, qnrB19, sul3, tet(A) | n.d | 10 |
20-MO00019 | 2018 | Pig, feces | AMP, CIP, FOT, GEN, SMX, TAZ, TET | aadA1, acrF, blaCTX-M-1, blaEC, blaTEM, emrD, mdtM, qnrB19, tet(A) | n.d | 10 |
20-MO00028 | 2017 | Poultry, feces | AMP, CIP, FOT, SMX, TAZ, TET | acrF, blaCTX-M-1, blaEC, emrD, mdtM, qnrB19, sul2, tet(A) | n.d | 3995 |
20-MO00045 | 2017 | Poultry, feces | AMP, CIP, FOT, SMX, TAZ, TET | aadA2, acrF, blaCTX-M-1, blaEC, emrD, lnu(F), mdtM, qnrB19, sul2, tet(A) | n.d | 3995 |
20-MO00078 | 2018 | Poultry, feces | AMP, CIP, FOT, NAL, SMX, TAZ, TET, TMP | aadA1, aadA5, acrF, aph(3'')-Ib, aph(6)-Id, blaCTX-M-1, blaEC, blaTEM-1, dfrA1, dfrA17, emrD, lnu(F), mdtM, mph(B), qnrB19, sul1, sul2, tet(A) | GyrA (S83L), ParE (I355T) | 4994 |
20-MO00080 | 2018 | Poultry, feces | AMP, CIP, FOT, GEN, NAL, SMX, TAZ, TET, TMP | aac(3)-IIe, aadA1, acrF, blaEC, blaSHV-2, blaTEM, dfrA1, emrD, lnu(F), mdtM, qnrB19, sul1, tet(A) | GyrA (S83L, D97N), ParC (S80I) | 533 |
14-AB01030 | 2016 | Poultry, feces | AMP, CHL, CIP, COL, SMX, TMP | aadA1, aadA2, acrF, blaEC, blaTEM-135, catA1, cmlA1, dfrA1, emrD, mcr-1.1, qnrB19, sul1, sul3, tet(M) | ParE (I529L) | 131 |
17-AB00065 | 2016 | Poultry, feces | AMP, CIP, GEN, TET | aac(3)-VIa, aadA1, acrF, blaEC, blaTEM-1, emrD, mdtM, qnrB19, tet(A) | n.d | 349 |
17-AB00089 | 2016 | Poultry, feces | CIP | acrF, blaEC, emrD, mdtM, qnrB19 | n.d | 69 |
17-AB00375 | 2017 | Pig, feces | CIP | acrF, aph(3'')-Ib, aph(6)-Id, blaEC, emrD, mdtM, qnrB19 | n.d | 117 |