Fig. 3

Identification of putative master regulators within the candidate region. To be considered as putative master regulator of reproductive polyphenism, such candidate(s) must display a different response to photoperiod between the CP and the OP genotype. First, these genes must be differentially expressed (DE) in the CP when submitted to photoperiod shortening. This information is indicated for each gene and for each time point within the squares of the column P (Photoperiod response in the CP). Red and green squares indicate genes up-regulated and down-regulated under SD, respectively. Second, these genes must also be DE between the CP and the OP lineage under SD photoperiod, which is necessary to induce the reproductive mode switch in the CP or reveal its inability in the OP. This information is compiled within the squares of the column L (Lineage response under SD). Yellow and blue squares indicate genes up-regulated and down-regulated in the CP, respectively. Finally, four genes fulfil these two conditions (black arrows) and thus represent strong candidates potentially responsible for reproductive mode variation between CP and OP lineages: LOC100168655 (Scavenger receptor class B member 1) in heads and LOC100159148 (Nuclear pore complex protein or Nup62), LOC100168027 (microprocessor complex DGCR8-like or pasha4), LOC100165999 (anaphase-promoting complex subunit 10 or APC10) in embryos. Genes that are not expressed or not DE in a particular condition are indicated with grey and black squares respectively. Genes are listed in the way they are ordered in the genome