Fig. 6

Relative expression of Msa-miR166s in Roots and leaves Note: 0 h: The experiment involved alfalfa plants that were not subjected to drought treatment, as well as those that were treated with drought for 6, 12, and 24 hours. Each group, both experimental and control, consisted of three pots of plants. The PEG group is represented by blue bars, the PTIO group by pink bars, and the SNP group by green bars. Error bars indicate the standard error (SE) of three biological and three technical replicates. Different letters indicate significant differences (p < 0.05) between samples, as determined by Tukey’s test. The values were normalized using the U6 gene