Mechanism of action of microRNA166 on nitric oxide in alfalfa (Medicago sativa L.) under drought stress

BMC Genomics

Table 2 Target genes and their cleavage sites corresponding to Msa-miR166s

Msa-miR166s	Target Gene	Transcript ID	TSlice	DegradomeCategory	DegradomePval
Msa-miR166a	ATHB-15	MS.gene069869	553	0	0.00045
	ATHB-8	MS.gene40724	556	0	0.00229
	REV	MS.gene08703	580	0	0.00504
Msa-miR166b	CoA ligase	MS.gene003303	1220	0	0.00549
	ATHB-15	MS.gene069869	553	0	0.00137
	ATHB-8	MS.gene40724	556	0	0.00229
	REV	MS.gene08703	580	0	0.00526
Msa-miR166c	CoA ligase	MS.gene003303	1220	0	0.00549
	ATHB-15	MS.gene069869	553	0	0.00137
	ATHB-8	MS.gene40724	556	0	0.00229
	REV	MS.gene08703	580	0	0.00526
Msa-miR166d	ATHB-8	MS.gene40724	556	0	0.00045
Msa-miR166d	REV	MS.gene08703	580	0	0.00343
Msa-miR166e-5p	FRI	MsG0880042046	1704	2	0.37533
Msa-miR166g-5p	SF1	MsG0480019689	1419	2	0.04909

TSlice is the binding site of miRNAs to the mRNA of the target gene, the 10th nucleotide at the 5’ end of the degradation fragment paired with miRNA; Degradome Category 0: The original data fragment is at this position, with an abundance equal to the maximum abundance on the transcribed RNA, and only one maximum value; Category 1: The abundance of the original data segment at this position is equal to the maximum abundance value on the transcribed RNA, and there is more than one maximum abundance value; Degradome Category 2: The abundance of the original data segment at this location is less than the maximum abundance on the transcribed RNA, but greater than the median abundance on the transcribed RNA

ISSN: 1471-2164