Fig. 5

Quantitative RT‒PCR (qRT‒PCR) validation of DEGs at different developmental stages (E21, E27, D0, and D7). The x-axis represents the sample name, and the y-axis represents the relative expression level (2-dd-Ct). Data are expressed as mean ± SD of three biological replicates. Different small letters above the bars indicate significant difference among the different sample groups (p < 0.05)