Fig. 7

Expression patterns of miR-301a-3p, ANKRD1 and Myogenic differentiation marker gene during satellite cell differentiation of Muscovy duck. Primary myosatellite cells were treated with myogenic differentiation medium (2% horse serum, 1% double antibodies, 1% GlutaMAX, 90% high-glucose DMEM) for 0, 2, 4, and 6 days, and the expression levels of miR301a-3p, ANKRD1 and the myoblast differentiation genes MyHC, MyoD, MyoG and Myf5. The data are expressed as the mean ± SD (n = 3). *P < 0.05 and **P < 0.01 for comparisons between groups