Fig. 5

Distribution of the transcripts in the four testicular cell populations. (A) Box plot of expression levels (log2FPKM) of all detected transcripts. All: all detected transcripts; aT: annotated transcripts; uT: unannotated transcripts; aT-COD: annotated coding transcripts; uT-COD: unannotated coding transcripts; aT-NONCOD: annotated noncoding transcripts; uT-NONCOD: unannotated noncoding transcripts. (B) Unannotated transcripts that were coincidentally identified as such with the four programs for coding potential analysis (and depicted in Fig. 4), distributed according to their expression in each of the four testicular cell populations. Transcripts are categorized into coding or noncoding, and transcripts of unannotated genes (of uG) or splice variants of already annotated genes (of aG). Note that many transcripts may be expressed in more than one stage. (C) Venn diagram indicating the distribution of the transcripts represented in B, in the four testicular cell populations. (D) DE coding and noncoding transcripts between pairwise sample comparisons of the four populations in chronological order. Of uG and of aG denote the same as in B