Fig. 1

RNA sequencing of human meniscus cells in osteoarthritis (OA) and healthy samples. (A) Volcano plots presenting all differential expressed genes (DEGs) between OA and healthy samples. false discovery rate (FDR) ≤ 0.01 and fold change ≥ 2 or ≤ 0.5. (B) Principal component analysis (PCA) based on fragments per kilobase of exon per million mapped fragments (FPKM) value of all DEGs. The ellipse for each group is the confidence ellipse. (C) Hierarchical clustering heatmap showing the expression of all DEGs. (D) Bar plot showing the most enriched GO pathways of the upregulated genes. (E) Bar plot showing the most enriched GO pathways of the downregulated genes. (F) Boxplot showing FPKM of 5 DEGs. ^*: p < 0.05, ^**: p < 0.01, ^***: p < 0.001