Fig. 4

Covariation analysis of differentially expressed RNA binding protein (DERBP) and regulatory alternative splicing (RAS) in apoptotic pathway of OA. (A) Venn diagram showing the overlap of RBP and DEG. (B) Heatmap showing the expression profile of DERBP in apoptotic pathway of osteoarthritis. (C) Covariation network of DERBPs and apoptosis-associated RAS (pSAR ≥ 50%). Cutoffs of p ≤ 0.01 and Pearson coefficient ≥ 0.6 ≤ -0.6 were applied to identify the covariation pairs. (D) The read distribution of the apoptotic RAS gene MTFP1 is showing in the left panel. The red box indicates the splicing area of the difference. The boxplots in the right panel show the FPKM of the coexpressed RBP gene AFF2 and the splicing ratio of MTFP1. ^*: p < 0.05, ^**: p < 0.01, ^***: p < 0.001. (E) The reads distribution of the apoptotic RAS gene CARD8 is showing in the left panel. The red boxes indicate the splicing area of the difference. The boxplots in the right panel show the FPKM of the coexpressed RBP genes LAMA2 and CUL4B and the splicing ratio of CARD8. ^*: p < 0.05, ^**: p < 0.01, ^***: p < 0.001