Fig. 1

Clustering of A. suumintestinal cells relative toC. elegansL2 whole-worm cells. a. A schematic describing the intestinal region comprising the ∼ 2/5th s of the whole intestinal length that was dissected for scRNAseq analysis. b. Bisbenzimide-stained adult female A. suum intestine image used to estimate total cell count per intestine. c. tSNE visualization of integrated single-cell data from A. suum untreated samples and the previously published single-cell C. elegans L2 whole worm sample, colored according to assigned cluster numbers. d. The same tSNE plot, recolored to instead distinguish A. suum intestine cells (red) from C. elegans cells, colored according to previously defined source tissues. e. The number and fraction of C. elegans cells in each cluster, colored by previously defined C. elegans tissue annotation. f. The same tSNE clustering showing only A. suum intestinal cells. The black border highlights the region containing clusters enriched in C. elegans intestine or not assigned (NA) cells, and 72.7% of all A. suum intestine cells. g. The same tSNE clustering showing only C. elegans whole L2 cells, labeled according to previously defined source tissues