Fig. 1

Sequencing, assembly, and features of the macronuclear genome of Pseudourostyla cristata. A Characteristics of all contigs and morphology of P. cristata. Ventral view in vivo is shown in a. Tracks b to f represent the distribution of gene density in sense strand (+), the distribution of gene density in antisense strand (-), GC content, genome coverage of reads, gene number of complete chromosomes, with densities calculated in 100-kb, 100-kb, 1-kb, 100-kb, 2-kb windows, respectively. All contigs are arranged from small to large in size (the outermost circle). B Statistics on assembly and annotation information of the macronuclear genome of P. cristata. Alveolate database (alveolata_odb10) was used for BUSCO analysis. C Distribution pattern of sequencing depth of all contigs. D The cumulative distribution of contig length. E The length distribution of contigs with different telomeres (0, 1 and 2). F The usage of three typical stop codons in P. cristata (Q, Glutamine; *, stop). G The length distribution of coding sequence (CDS). H The length distribution of intron sequence. Motifs of 31 and 34 bp introns are listed on the top right