Fig. 7

Consensus tree based on eighty-six chitin synthases (CHS) proteins from nine ciliates and six CHS proteins from two fungi, with corresponding information of gene expression, motif, conserved domain, and gene structure. A Normalized gene expression level of different CHS genes. Data are represented as log10-transformed ratios of TPM values [log10(TPM value)]. Grey dots at the vertical axis indicate corresponding genes not supported by RNA-seq reads. B Consensus tree inferred from 86 CHS proteins of nine ciliates and six CHS proteins of two fungi. Sc, Stentor coeruleus; Bs, Blepharisma stoltei; Ev, Euplotes vannus; Sl, Stylonychia lemnae; Ot, Oxytricha trifallax; Pc, Pseudourostyla cristata; Tt, Tetrahymena thermophila; Pp, Pseudocohnilembus persalinus; Pt, Paramecium tetraurelia. Elliptical and rectangular profiles represent that the pellicle of ciliates is soft and rigid, respectively. Black circles indicate that node support values greater than 95% (high confidence value). C MEME motif distribution of each protein. D Conserved domain of each protein. E Corresponding gene structure of each CHS protein. The green boxes in (E) represent exons, lines represent introns. The four boxes on the black background below the figure are the sum of TPM for CHS genes in each species and the legends of (C), (D), and (E), respectively