Fig. 1

The gene expression signature of Daphnia mitsukuri during development. A Diagram representing the experimental design. Three developmental stages (three replicates of each time point) were selected: embryo (E: 6–8 h after oviposition); juvenile (J1: 2 days old after birth; J2: 4 days old; J3: 6 days old), and adult (A: 8 days old). Images of the studied developmental stages, scale bars in each image represent 200 μm. B Principal-component analysis of normalized gene expression counts for D. mitsukuri samples from three developmental stages. The plot of the first two principal components is shown. C Heat map of hierarchical clustering performed on Pearson correlations, calculated using normalized gene expression counts, between each pair of samples from three developmental stages (embryo, juvenile and adult). D Numbers of up- and down-regulated genes based on adjacent pairs of age groups. A gene with FDR-corrected P-value < 0.05 and FC ≥ 6 or was considered as up-regulated and a gene with FDR-corrected P-value < 0.05 and FC ≤ -6 was considered as down-regulated