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Table 1 In-house mock communities’ components and genomic characteristics

From: Full-length 16S rRNA gene sequencing by PacBio improves taxonomic resolution in human microbiome samples

Mock 1

DSM

Name

Refseq

16S rRNA gene copy number

Genome size (bp)

Mass of DNA per genome1

Nº genomes per ng

Nº 16S rRNA gene copies per ng

RA2 (%)

DSM 23056

Actinomyces oris

NZ_CP066060.1

3

3,184,721

3.436E-06

2.91 × 105

8.73 × 105

12.05

DSM 20482

Fusobacterium nucleatum

NZ_LN831027.1

5

2,443,126

2.636E-06

3.79 × 105

1,9 × 106

26.19

DSM 43762

Rothia dentocariosa

NC_014643.1

3

2,506,025

2.704E-06

3.7 × 105

1.1 × 106

15.32

DSM 20627

Streptococcus oralis

NZ_LR134336.1

4

1,931,548

2.084E-06

4.8 × 105

1.9 × 106

26.50

DSM 17713

Neisseria sicca

NZ_CP072524.1

4

2,566,407

2.769E-06

3.61 × 105

1.44 × 106

19.94

      

Total

7.2 × 106

100

Mock 2

DSM

Name

Refseq

16S rRNA gene copy number

Genome size (bp)

Mass of DNA per genome1

Nº genomes per ng

Nº 16S rRNA gene copies per ng

RA2 (%)

DSM 23056

Actinomyces oris

NZ_CP066060.1

3

3,184,721

3.436E-06

2.91 × 105

8.73 × 105

10.79

DSM 20482

Fusobacterium nucleatum

NZ_LN831027.1

5

2,443,126

2.636E-06

3.79 × 105

1,9 × 106

23.44

DSM 43762

Rothia dentocariosa

NC_014643.1

3

2,506,025

2.704E-06

3.7 × 105

1.1 × 106

13.71

DSM 20627

Streptococcus oralis

NZ_LR134336.1

4

1,931,548

2.084E-06

4.8 × 105

1.9 × 106

23.71

DSM 20627

Streptococcus mutans

NZ_LS483349.1

5

2,019,343

2.178E-06

4.6 × 105

2.29 × 106

28.35

      

Total

8.09 × 106

100

  1. 1Mass of DNA per genome. Number of genome bp * weigh of a bp (1.079 × 10–12 ng/bp)
  2. 2RA = Estimated relative abundance in percentage