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Table 2 Number of reads sequenced and annotated by platform and sample type

From: Full-length 16S rRNA gene sequencing by PacBio improves taxonomic resolution in human microbiome samples

Platform

Sample Type

Nº Reads Mean (SD)

Filtered1 Mean (SD)

Reads length mean

Total ASV2

Total Genera2

% reads assigned to Genera

% reads assigned to Species

Illumina

Mock 1

91,804 (16,260)

75,620 (13,350)

417.5 bp

7

6

100

58.33

Mock 2

90,301 (856)

62,444 (44)

419.5 bp

15

5

100

69.27

Saliva

94,079 (14,632)

70,661 (10,323)

418.1 bp

1414

138

96.46

52.79

Subgingival

110,328 (44,092)

84,896 (36,315)

415.3 bp

1328

153

95.5

55.73

Faeces

212,795 (122,662)

157,113 (94,087)

408.5 bp

1113

201

89.49

53.13

PacBio

Mock 1

26,065 (1431)

15,536 (870)

1455.5 bp

14

5

100

100

Mock 2

33,620 (7213)

19,821 (2565)

1469 bp

17

4

99.99

99.73

Saliva

21,151 (12,907)

17,444 (11,848)

1459.33 bp

2247

113

97.25

74.91

Subgingival

13,982 (2163)

10,103 (2796)

1456.44 bp

1380

99

95.41

72.80

Faeces

12,702 (2185)

7350 (2312)

1452.25 bp

796

97

89.72

61.93

  1. 1Filtered refers to number of sequences after quality check, primer trimmed, merged (only for Illumina dataset) and chimeric removal
  2. 2Total detected, excluding those with a number of reads < 10