Fig. 5

Expression analysis of selected GgABCB subfamily genes in shoot and root tissues under phytohormonal (a to d) and N-1-Naphthylphthalamic acid treatment (e). GgABCB genes are present in rows and treatment time duration in columns of the matrix. Heat map showing-Cluster analysis of GgABCB genes according to their expression profiles in Naphthaleneacetic acid (NAA) treated (a) shoots, (b) roots; and Methyl jasmonate (MeJA) treated (c) shoots, (d) roots for 0, 4, 8, 16, 32, 64 and 128 h time interval; (e) qRT-PCR expression (fold) represented in the form of bar graph of the PIN1, PIN3/4/7, TWD1 (TWISTED DWARF1) and selected ABCB genes under the influence of NPA (N-1-naphthylphthalamic acid) post 15 days of treatment. Actin was used as an internal reference. Three biological replicates were used to calculate error bars using standard deviation. The Y-axis indicates relative expression level and X-axis indicates shoot/root tissues. Statistical significance was determined using One-way ANOVA in Graphpad prism 8.0 software. When compared with the control, single asterisk shows a significant difference with a P-value < 0.05. Double asterisks show a significant difference with a P-value < 0.01, three asterisks show a significant difference with a P-value < 0.001 and four asterisks shows a significant difference with a P-value < 0.0001