Fig. 3
From: Identification and functional analysis of the LEAFY gene in longan flower induction
Subcellular localization and transactivation analysis of DlLFY. (A) Subcellular localization of the DlLFYGFP fusion protein in Arabidopsis protoplasts. The fluorescence signals of GFP (Green fluorescent protein), NLS (Nuclear locating sequence) and Chloroplast in protoplasts were observed at a wavelength of 488 nm, 561 and 640 nm, respectively. Scale bar = 10 μm. (B) Transactivation activity analysis of full-length DlLFY in yeast. The initial concentration of yeast was adjusted to an OD600 of 0.1 and diluted 1/10, 1/100. Negative control, pGBKT7; positive control, pGBKT7-53 + pGADT7-T