Fig. 3
From: Identification of different myofiber types in pigs muscles and construction of regulatory networks
ceRNA network construction and functional enrichment analysis of the target genes. (A) Venn diagram showing the overlap of DEmiRs identified by small RNAseq quantification and RNA seq-based prediction. (B) Target gene prediction of 25 miRNAs. (C) The lncRNA‒miRNA‒mRNA ceRNA network. Yellow triangles, pink circles and purple squares indicate mRNAs, lncRNAs and mRNAs, respectively. The node size reflects the degree of the same kind of molecule. (D) Dual-luciferase reporter assay of wild-type (WT) plasmids and mutantion (MUT) plasmids. Sequence alignment of miR-24-3p with the SMIM8 WT sequence and MUT sequence. (E) Relative Renilla luciferase activity analysis after transfection with NC mimics + SMIM8 WT plasmids, miR-24-3p mimics + SMIM8 WT plasmids, NC mimics + SMIM8 MUT plasmids, and miR-24-3p mimics + SMIM8 MUT plasmids (n = 6). (F) Dual-luciferase reporter assay of WT sequence and MUT plasmids. Sequence alignment of miR-24-3p with the MAVS WT sequence and MUT sequence. (G) Relative Renilla luciferase activity analysis after transfection with NC mimics + MAVS WT plasmids, miR-24-3p mimics + MAVS WT plasmids, NC mimics + MAVS MUT plasmids, and miR-24-3p mimics + MAVS MUT plasmids (n = 6). (H) Bar plot of the results of KEGG enrichment analysis of the target genes. (I) Chord plot of the targeted genes and associated Gene Ontology pathways