Fig. 4
From: Identification of different myofiber types in pigs muscles and construction of regulatory networks
Expression of the miR-10 family in LD and SOL. (A) Schematic representation of the mir-10 family in S. scrofa. The miRNAs of the miR-10 family were grouped into two distinct genomic clusters based on different genomic locations and four functional clusters based on seed sequence similarities. (B) Heatmap of miR-10 family expression in LD and SOL cells. The data are presented as lgRPM values extracted from the small RNA-seq dataset. (C) Differential expression of miR-10 cluster-associated miRNAs in LD and SOL samples. (D) Dual-luciferase reporter assay of WT plasmids and MUT plasmids. Sequence alignment of miR-10a-5p with the MYBPH WT sequence and MUT sequence. (E) Relative Renilla luciferase activity analysis after transfection with NC mimics + MYBPH WT plasmids, miR-10a-5p mimics + MYBPH WT plasmids, NC mimics + MYBPH MUT plasmids, and miR-10a-5p mimics + MYBPH MUT plasmids (n = 6). (F) Expression levels of miR-99b, miR-125a, miR-378, miR-10a-3p, miR-10a-5p and miR-24-3p in the LD and SOL muscles (n = 4). The results are presented as the means ± SEMs. The significance of differences between means was analyzed via an independent sample t test (*P < 0.05; **P < 0.01)