Fig. 2From: Common occurrence of hotspots of single strand DNA breaks at transcriptional start sitesSSB hotspots are significantly enriched around TSSs in different tissue types. (A) Aggregate plots of the normalized density scores (Y-axes) of the positions of all SSBs and various SSB hotspots within ± 5,000 bp of TSSs in heart. The opaque vertical rectangles represent the ± 200 bp regions around the TSSs. The signal from the template and nontemplate strands is shown respectively above and below the zero lines. (B, C) The TSS-SSBs enrichment ratios (Y-axes) for all SSBs (background) and SSB hotspots on either template or non-template strands for different tissues: heart and liver (B) and the other four tissues (C). Yellow represents background (all SSBs), red represents sample-level hotspots (depth ≥ 2), blue represents sample-shared hotspots (depth ≥ 1) and purple represents sample-shared hotspots (depth ≥ 2). Note, two data points in sperm for the sample-shared hotspots (depth ≥ 1) and background have the same values (Supplementary Table 3), thus only the latter is shownBack to article page